anti tm4sf1 pe antibody (Miltenyi Biotec)
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Miltenyi Biotec
anti tm4sf1 pe antibody
Anti Tm4sf1 Pe Antibody, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tm4sf1+antibody/TM4SF1+Antibody%2C+anti-human%2C+REAfinity/pmc12174346-262-17-19
Average 93 stars, based on 6 article reviews
Anti Tm4sf1 Pe Antibody, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tm4sf1+antibody/TM4SF1+Antibody%2C+anti-human%2C+REAfinity/pmc12174346-262-17-19
Average 93 stars, based on 6 article reviews
anti tm4sf1 pe antibody - by Bioz Stars,
2026-11
93/100 stars
Images
1) Product Images from "Bladder cancer variants share aggressive features including a CA125+ cell state and targetable TM4SF1 expression"
Article Title: Bladder cancer variants share aggressive features including a CA125+ cell state and targetable TM4SF1 expression
Journal: Nature Communications
doi: 10.1038/s41467-025-59888-8
Figure Legend Snippet: A Volcano plot comparison of all UC and HV cells after downsampling ( N = 150 cells per patient) ( p -values are computed from the two-sided Wilcoxon rank-sum test). B Violin plots of TM4SF1 expression by tumor cluster. Source data are provided as a Source data file. C , D Immunohistochemistry of TM4SF1 in a validation cohort of HV and UC C primary tumors and D lymph node metastases. E Semiquantitative comparison of TM4SF1 staining in HV and UCs (Fisher’s exact test, p = 0.02). Scale bars = 50 µm.
Techniques Used: Comparison, Expressing, Immunohistochemistry, Biomarker Discovery, Staining
Figure Legend Snippet: A Quantification of in vitro TM4SF1-CAR1 and CAR2 activity against bladder cancer cell lines using IncuCyte co-culture assay with 1:1 effector:target cell ratio. B In vitro co-culture assays comparing CAR activity (2:1 effector:target cell ratio) in UMUC3 cell lines with and without CRISPR-mediated TM4SF1 knockout ( N = 1 replicate per condition, 5 conditions per experiment). C Evaluation of CAR1 tumor-killing efficacy in an in vivo xenograft model using UMUC3 ( N = 9 mice) and 253JBV ( N = 10 mice) cell lines. Data are presented as mean values ± standard error bars. D Kaplan–Meier survival analysis of CAR1 treated and untreated mice with UMUC3 ( N = 9 mice) and 253JBV ( N = 10 mice) tumors. E:T Effector:Target, UTD untransduced, CAR chimeric antigen receptor.
Techniques Used: In Vitro, Activity Assay, Co-culture Assay, Co-Culture Assay, CRISPR, Knock-Out, In Vivo
Related Articles
Staining:Article Title: Histologic variants in bladder cancer harbor aggressive molecular features including TM4SF1 expression and a CA125+ cell state Article Snippet: 48–72 h after transfection, GFP-positive cells were sorted by FACS (BD Fusion) and expanded. .. Cells were then stained with a Article Title: Bladder cancer variants share aggressive features including a CA125+ cell state and targetable TM4SF1 expression Article Snippet: 48–72 h after transfection, GFP-positive cells were sorted by FACS (BD Fusion) and expanded. .. Cells were then stained with a Article Title: Bladder cancer variants share aggressive features including a CA125+ cell state and targetable TM4SF1 expression. Article Snippet: UMUC-3 TM4SF1-KO cells were generated by transient transfection (Lipofectamine 3000) ofUMUC-3 cells with PX458 (Addgene, #48138). .. Cells were then stained with a FACS:Article Title: Histologic variants in bladder cancer harbor aggressive molecular features including TM4SF1 expression and a CA125+ cell state Article Snippet: 48–72 h after transfection, GFP-positive cells were sorted by FACS (BD Fusion) and expanded. .. Cells were then stained with a Article Title: Bladder cancer variants share aggressive features including a CA125+ cell state and targetable TM4SF1 expression Article Snippet: 48–72 h after transfection, GFP-positive cells were sorted by FACS (BD Fusion) and expanded. .. Cells were then stained with a Article Title: Bladder cancer variants share aggressive features including a CA125+ cell state and targetable TM4SF1 expression. Article Snippet: UMUC-3 TM4SF1-KO cells were generated by transient transfection (Lipofectamine 3000) ofUMUC-3 cells with PX458 (Addgene, #48138). .. Cells were then stained with a |
![<t>TM4SF1</t> expression in the endothelium in vivo . TM4SF1 IHC staining with HRP-conjugated 2nd antibody and eosin was conducted in tissue sections prepared from ( A ) 8 week old C57BL/6 mouse organs [liver ( a ), kidney ( b ), heart ( c ), lung ( d ), and brain ( e )] and ( B ) human liver. Wholemount staining was performed in postnatal day 18 C57BL/6 mouse retina ( f ) with Alexa488-conjugated anti-mouse TM4SF1 antibody <t>2A7A.</t> Representative IHC staining in both ( A , B ) tissue sections showed that TM4SF1 expression was largely limited to blood vessels with a tendency for the highest TM4SF1 expression to be in arteries (A), intermediate expression in veins (V), and the lowest expression in capillaries (C). Positive TM4SF1 expression was also seen in kidney glomeruli (Gm) and in the choroid plexus (CP; black arrow), the epithelium of lateral ventricles (LV), along with the subependymal cell layer (SEL) in the brain; no expression was observed in the cortex (CX) and thalamus (Th). ( C ) qPCR using total RNA (normalized against 10 6 18S copies) acquired from three different human aorta and pulmonary vein samples showed that TM4SF1 expression was 4.8-fold higher in the artery than in the vein ( p = 0.0026).](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_2815/pmc11592815/pmc11592815__cimb-46-00781-g001.jpg)
